Characterization of Two Anti-Human Parathyrin Antisera
نویسندگان
چکیده
منابع مشابه
In vitro characterization of anti-glucosylceramide rabbit antisera.
Glucosylceramides (GlcCer) are biosynthetic precursors of glycosphingolipids. They are widely distributed in biological systems where they exhibit numerous biological functions. Studies on the localization of glucosylceramides in different tissues have used biochemical methods only since specific antibodies against GlcCer were not previously available. We have characterized two commercially ava...
متن کاملParathyrin radioimmunoassay: diagnostic utility of antisera produced against carboxyl-terminal fragments of the hormone from the human.
Antisera directed toward the carboxyl-terminal region of human parathyrin (parathyroid hormone), for use in daignostically applicable radioimmunoassays of the hormone in serum, are scarce, largely because of the lack of suitable immunogens of human origin. We produced four antisera in goats and guinea pigs by immunization with recently discovered carboxyl-terminal fragments of human parathyrin ...
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This is an immunoradiometric assay of intact human parathyrin, hPTH(1-84). One antibody, directed against the N-terminal part of the hormone, was produced in goats and conjugated covalently to cellulose particles. hPTH(1-84) and the N-terminal fragments were extracted from EDTA-treated plasma by these particles and thus concentrated. Another antibody, against synthetic hPTH(53-84), was raised i...
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BACKGROUND Respiratory syncytial virus (RSV) infection is a major problem in the newborn and aging populations. Fully human monoclonal antibodies with the ability to neutralize RSV could have a major impact on the immunotherapy of the disease. The generation of human antibodies has been difficult because there exists no general way to activate B cells against an antigen of choice in vitro. MA...
متن کاملImmunologic and Biologic Characterization of Antisera
Sensitive immunologic techniques and more purified preparations of bovine pituitary thyrotropin have made it possible to re-examine the question of whether thyrotropin acts as an antigen upon injection into heterologous species. This possibility was first indicated by Werner (1, 2) in 1936, to explain the loss of responsiveness to thyrotropin which Collip and Anderson (3) previously had observe...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1982
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1982.20.4.175